I have a head-scratcher of a problem. I have kept tropical fish almost continuously for over 60 years. and consider myself reasonably experienced. I currently have 240, 125, 70 , 50 and 29 gallon tanks as well as a 1,000 gal aquaponic system. My problem is with the 240 which has been set up for over 10 years. Inhabitants are a large aro, large sev, 3 large silver dollars , a large flag tail prochilodus, and 2 medium geos. I have 2 large canisters and a 2-gal/hour continuous spring water drip with a wave maker for circulation. Ammonia and nitrites are zero, nitrates < 20. Temp 80 degrees, White filter sand substrate and lots of driftwood with biofilm. I feed daily aro pellets, bug bites and pleco wafers. All fish are healthy and several years old. The problem: any pleco I add dies within a few days. I have lost gibbiceps, common pleco, bristlenose and a feather fin squeaker. My recent heartbreaking experience was when moving a 7" Royal pleco from my 70, along with 2 geos. I drip acclimated them for an hour, even though parameters were apparently the same. The geos are doing well but, as with the others, the Royal died within 3 days for no obvious reason. No bullying., no gasping at the surface, etc. Could it be something in the substrate (which I stir occasionally), or the biofilm? The flag tail grazes some and the geos sift the sand with no ill effects. I'm considering replacing all substrate and decorations but I wonder if anyone has any other ideas? I've never had a problem like this. Help please!