The data on thiaminase content comes from various sources, mostly from the National research council (1982), Deutsch & Hasler (1943), Greig & Gnaedinger (1971) and Hilker & Peter (1966); see also the literature list at the end of the article. The lists are far from complete, but most of the usually marketed and so far examined species are enlisted. Although primarily based on coldwater food fish and invertebrates, thiaminase content information exists for several tropical species widely marketed, and these been included accordingly.
Species that contain thiaminase
Freshwater fish
Family Cyprinidae (Minnows or carps):
Common bream (Abramis brama)
Central stoneroller (Campostoma anomalum)
Goldfish (Carassius auratus)
Common carp (Cyprinus carpio)
Emerald shiner (Notropis atherinoides)
Spottail shiner (Notropis hudsonius)
Rosy red, Fathead minnow (Pimephales promelas)
Olive barb (Puntius sarana)
What is thiaminase?
Thiaminase is an enzyme, a chemical compound that destroys or inactivates thiamine. Thiamine is an important vitamin also known as Vitamin B1. There is not just one type of thiaminase, but several different ones, some of which can be produced by bacteria, fungi, plants and potentially animals.
hiamine Deficiency Syndrome and its symptoms
Vitamin B1 (also known as thiamine, thiamine hydrochloride, and in older text books, as aneurine hydrochloride) is an essential nutrient for most animals. It is a colorless and water soluble chemical that helps to convert carbohydrates into glucose. It is particularly important for the correct functioning of the nervous system. A lack of Vitamin B1 is called a Thiamine Deficiency Syndrome.
Symptoms of this syndrome are well known from several commercially important fish groups and can be confirmed using appropriate biochemical tests. Flatfish fed exclusively with thiaminase-rich clams suffer and die from paralysis and related physical shocks. Eels show a trunk-winding syndrome and hemorrhages along the base of the fins (similar symptoms have been reported from moray husbandry, too). Salmonids show nervous disorders, poor appetite, poor growth and jumpiness (again, similar things have observed among a variety of ornamental fish species). Skin congestion and haemorrhage have been reported from carp and other cyprinids. In general then, excessive amounts of thiaminase are connected with symptoms of sickness that include poor growth, loss of appetite, abdominal swelling and hemorrhage, loss of equilibrum, convulsions, muscle atrophy and a weak immune system.
While it has not yet been scientifically proven that pet fish suffering from the above mentioned symptoms have Thiamine Deficiency Syndrome, the parallels with their food fish relatives are striking. The problems of thiaminase are now well known in the professional fields of animal nutrition (e.g. fish farms), but so far this information has not been widely taken up by aquarists and pet owners. But it is clear that those hobbyists keeping large predatory fish and other carnivorous animals need to be familiar with the problem of Thiamine Deficiency Syndrome, and use that information to make sensible choices when selecting food for their livestock.
References
Anglesea, J.D. & Jackson, A.J. (1985): Thiaminase activity in fish silage and moist fish feed. Anim. Feed Sci. Tech. 13: 39-46.
Deutsch, H.F. & Hasler, A.D. (1943): Distribution of a Vitamin B1 destructive enzyme in fish.- Proc. Soc. Exp. Biol. Med. 53: 63-65.
Food and agriculture organization of the United Nations (1980): ADCP/REP/80/11 - Fish Feed Technology. HYPERLINK "http:]www.fao.org/docrep/X5738E/x5738e00.HTM#Contents" http:]www.fao.org/docrep/X5738E/x5738e00.HTM#Contents
Greig, R.A. & Gnaedinger, R.H. (1971): Occurrence of thiaminase in some common aquatic animals of the United States and Canada. Special Scientific Report—Fish. U.S. Dept. Commer. Natl. Mar. Fish. Serv. 631: 1-7.
Hilker, D.M. & Peter, O.F. (1966): Anti-thiamine activity in Hawaii fish.- J. Nutr. 89(4):419-421.
National Research Council (1981): Nutrient Requirements of Cold-water Fishes. Washington, D.C.: National Academy Press.
National Research Council (1982): Nutrient Requirements of Mink and Foxes, Washington, D.C.: National Academy Press.
National Research Council (1983): Nutrient Requirements of Warm-water Fishes and Shellfishes. Washington, D.C.: National Academy Press.
National Research Council (1993): Nutrient Requirements of Fish. National Academy Press. Washington DC, USA.
Royes, J.-A.B. & Chapman F.A.: Preparing your own fish feeds.- University of Florida, 9 p. HYPERLINK "http:]edis.ifas.ufl.edu/pdffiles/FA/FA09700.pdf" http:]edis.ifas.ufl.edu/pdffiles/FA/FA09700.pdf
Scardi, V. & Magri, E. (1957): Thiaminase activity in Mytilus galloprovincialis.- Boll Soc Ital Biol Sper. 33(7):1087-1089 (in Italian).
Wistbacka, S.; Heinonen, A.; Bylund, G. (2002): Thiaminase activity of gastrointestinal contents of salmon and herring from the Baltic Sea.- Journal of Fish Biology 60(4), 1031-1042.
Yudkin, W.H. (1949): Thiaminase, the Chastek-paralysis factor.- Physiol. Rev. 29: 389-402.
Neale Monks