bugs in a bottle...?

RD.

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I have read that thread in the past, the real irony is that at the end of that long winded discussion they are still arguing. lol I'm more of a hands on type of guy, and while tests & studies & papers are interesting, I began testing these products on my own years ago. Controlled? Not really. Peer reviewed? Nope. But good enough for me to understand that under the conditions that I used them, they worked. In fact I was using, and promoting septic tank bacteria (heterotrophs) for cycling many years ago, because just like discussed in the link that squint posted I knew that products like SeptoBac would consume ammonia, and assist in the cycle. Even Fritz admits that.

"In the absence of an organic nitrogen source, many heterotrophs can utilize ammonia instead."
The part that they left out in their little story, is that when cycling a bare bones new tank that is "fishless", and simply adding a source of liquid ammonia, those bacteria are forced to feed off that ammonia. And as squint knows, nitrifying bacteria are already in our water distribution lines, so they begin slowly building in the new tank as well. No study required, been there done that, as have many others, many years ago. A little trick that started approx 25 yrs ago with a now defunct cichlid club in Eastern Canada. (CRLCA)


There are a LOT of variables involved in these types of discussions, size of tank, pH, water temp, 02 levels, type of bio-media, the list goes on & on. One thing that I discovered with Seachem Stability, and my set up, was that in order to maximize the speed of the cycle, I had to use double the Seachem recommended dose. A tank that was showing high ammonia levels (I had wiped out most of the bio-bacteria from the use of medication) was brought back to a fully cycled balanced tank in 5 days. And from day 1, while using a double dose, the free ammonia level dropped to near 0, and I left the fish in for the full 5 days with no issues. Without Stability the fish would have most likely been scorched to death from the ammonia, before the bio bacteria recovered on their own.

What the exact science is in all of this I do not know, nor do I really care. I just know that many of these products do in fact work, exactly as claimed. Fritz isn't the only company that figured out how to keep these bacteria dormant in a liquid state. Anyone with a lab at their disposal can reverse engineer these products, and with a few tweaks take out their own patent. The dry formulas, such as the septic products, they are old school for sure, but they still have a place in this hobby as well. Different bacteria, for different measures.
 

jjohnwm

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Thanks for that link, S squint . Closing in on 1800 posts! I've started reading it; I think that if I set up a brand new tank right now, with no inoculation of anything other than ammonia...it might be properly and completely cycled by the time I get to the end of the thread. :)
 
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RD.

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Just to add to this, from this old paper that was written to promote Fritz products, they do cite a reputable source regarding the survival of these bacteria, while stored in a liquid state.


Products containing Nitrosomonas or Nitrobacter spp. cannot undergo this drying process because it would destroy the bacteria, however in liquid formulations the nitrifiers can survive as viable inactive cells which can be reactivated during periods of mineralization (Verhagen, et al, 1992).


That paper from 1992 is cited by numerous researchers over the years.
 
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deeda

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Just an update on the talk we attended re: Fritz Aquatics bacterial products. Jay talked about 4 products they offer for freshwater use, 2 nitrifying or Autotrophs and 2 sludge reducers or Heterotrophs.

Fritz Zyme 7 is nitrifying bacteria, buffered and does not need to be refrigerated. Fritz Zyme Turbo Start 700 is a nitrifying bacteria, must be refrigerated and has a shelf life of 6 months (different shelf life than website). Both products are Nitrosonomos and Nitribacter but did not elaborate on species. The difference between the 2 products is the latter has more nitrifying bacteria present.

Fritz Zyme 360 and Monster 360 are sludge reducers or degraders with Bacillus sp. and others. The difference between the 2 products is the latter is more concentrated.

It was an interesting talk and what I found odd was the suggestion that the more frequent or larger the water changes, the less Heterotrophic bacteria available and the more sludge build up in filters. I didn't get a chance to ask for clarification on this due to the number of members asking questions.

I did ask the following questions though:
Are the bacteria they produce in suspended animation? The answer was no.

Are the bacteria cultured? The answer was yes.

Is the pH reduced when bottled so they hibernate? The answer was no, they are all natural, just bacteria.

What about S. bacillus strains found in low-cost septic cleaning products (heterotrophs)? The answer was yes they are similar.
 

RD.

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Hi Dee, thanks for posting that. As far as I know, Jay doesn't work for Fritz, so perhaps his facts weren't 100% accurate? That's my polite way of addressing some of what he stated. :)

My understanding with some of the nitrifying products out there, is that 02 levels are reduced, which puts the bacteria in a state of "hibernation", for lack of a better word. Active "live" bacteria require food to survive, so I'm not sure how anyone can put nitrifying bacteria in a bottle, and expect them to live for months on end, without eating. That kind of makes sense as to why the bottle that contains higher levels of nitrifying bacteria, requires refrigeration. (to slow down their metabolism, hence their food requirement) IMHO, they have to be in a state of hibernation, or they wouldn't survive long in a small container. Heterotrophic bacteria can survive for years, in a dry state, as spores.

It was an interesting talk and what I found odd was the suggestion that the more frequent or larger the water changes, the less Heterotrophic bacteria available and the more sludge build up in filters. I didn't get a chance to ask for clarification on this due to the number of members asking questions.
Heterotrophic bacteria will survive, and multiply, VERY rapidly compared to nitrifying bacteria, such as Nitrosomonas or Nitrobacter spp, and they able to tolerate a much wider range of environmental conditions. But in order to survive they also require a food source, which in this case is mostly organics. They certainly don't just live in the water, so frequency or size of water changes does not even enter into the equation.

Typically once the organic sludge is utilized as a food source, heterotrophic bacteria turn on themselves for food, consuming each other, and soon after die off. They are apparently not very efficient at utilizing ammonia as a food source, so once the organics are consumed, they die off. This is precisely why one has to periodically re-dose heterotrophic bacteria in the aquarium. This is also why Fritz recommends to add a new dose every two weeks. https://fritzaquatics.com/products/fritzpond-fritzzyme-360

As you know I am testing out a new septic bacteria product, and dosing my 125's with 1/4 teaspoon weekly. I'll update that thread when I pull my filters for cleaning. https://www.monsterfishkeepers.com/forums/threads/the-use-of-probiotics-in-aquaculture.536531/
 

Wharf

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Interesting read so far...do you think the contents of Fritz 360 & Monster are probably the same or similar to Dr. Tim's Ecobalance...cause I bought that stuff when it was being sold for 3 dollars on Amazon for the 16 oz lol..(.think it was a listing error but basically got 48 oz for around 13 something dollars woops) after its done I probably will buy a small container of bio clean or ridx.
 

RD.

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All of the sludge reducing products are very similar, a mix of heterotrophic bacteria, along with certain enzymes such as amylase, cellulase, lipase and protease. The latter utilized in the breakdown of protein, fat, carbohydrates, and cellulose.
 

RD.

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Wharf Wharf - Dr. Tim's sludge reducer is called Waste Away.

No idea what the probiotic blend is that he is using in his Ecobalance, but most of the sludge reducers are also probiotic, and can be used for competitive exclusion, to keep pathogenic bacteria in check.
 
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Wharf

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Wharf Wharf - Dr. Tim's sludge reducer is called Waste Away.

No idea what the probiotic blend is that he is using in his Ecobalance, but most of the sludge reducers are also probiotic, and can be used for competitive exclusion, to keep pathogenic bacteria in check.
So do you still think Ridx, Bio-clean etc. is the most bang for your buck regarding heterotrophic flora?
 
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