If you mean by the injection, then Jello has never been injected, so far we injected two gulpers, two TSNxRTC hybrids, and one RTC. I gave a large excess, like 10x-100x excess, since these injections were meant to save their lives. Since B1 is highly water soluble, the excess is simply excreted I am told by our vet advisers.thebiggerthebetter maybe I missed it. How do you dose vit b1?